Simultaneous Determination of Baicalin,Linarin,Rhein in Cuochuang Xiaoyan Lotion by HPLC Method
10.3870/j.issn.1004-0781.2017.11.021
- VernacularTitle:高效液相色谱法同时测定痤疮消炎洗剂中黄芩苷蒙花苷大黄酸含量
- Author:
Jiangping ZHENG
1
;
Zhengde YE
;
Youlin ZHANG
;
Liangyong HUANG
Author Information
1. 湖北医药学院附属十堰市太和医院药学部
- Keywords:
Cuochuang Xiaoyan Lotion;
Chromactography;
high performance liquid;
Determination;
Quality standard
- From:
Herald of Medicine
2017;36(11):1294-1297
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a specific HPLC method for simultaneous determination of three components ( baicalin, linarin and rhein) in Cuochuang Xiaoyan lotion. Methods The three components in Cuochuang Xiaoyan lotion were assayed by HPLC gradient elution method.The assay was performed with Waters Xterra MS C18(4.6 mm×250 mm,5 μm) column, with acetonitrile (A) and 0.2% phosphoric acid solution (B) as mobile phase in gradient elution.The flow rate was 1.0 mL·min-1.The detection wave length was 277 nm and column temperature was 30 ℃. Results Three components were completely separated from the adjacent peaks and a good linear relationship between each sample concentration and integral area was obtained.The linear equations were as follows:Ybaicalin=9.208X-0.0994(R2=0.9999, 83.97-839.70 μg·mL-1);Ylinarin=3.0628X-0.0038 ( R2 = 0. 9999, 34. 75-347. 49 μg · mL-1 );Yrhein = 1. 0225X-0. 0286 ( R2 = 0. 9998, 63. 20-632.00 μg·mL-1 ) . Conclusion The HPLC method is simple, accurate and reproducible, which is effective in controlling the quality of Cuochuang Xiaoyan lotion.
