Analysis of HPLC Fingerprints and Determination of Nardosinone of Nardostachyos Radix et Rhizoma from Nardostachys jatamansi DC
10.3870/j.issn.1004-0781.2017.11.022
- VernacularTitle:甘松药材高效液相色谱指纹图谱及甘松新酮含量测定
- Author:
Maitinuer MAIWULANJIANG
1
;
Tingxia DONG
;
Huaqiang ZHAN
;
Aisa HAJIAKBER
Author Information
1. 中国科学院新疆理化技术研究所新疆特有药用资源利用重点实验室
- Keywords:
Nardostachyos Radix et Rhizoma;
Nardosinone;
Chromatography;
high performance liquid;
Quality control;
Fingerprint
- From:
Herald of Medicine
2017;36(11):1298-1302
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for quality evaluation of Nardostachyos Radix et Rhizoma derived from Nardostachys jatamansi DC by determining the contents of nardosinone and establishing fingerprints. Methods HPLC fingerprint and content determination methods were established to evaluate ten batches of Nardostachyos Radix et Rhizoma from Nardostachys jatamansi DC.For the determination of nardosinone, the samples were separated by Phenomenex-C18 ( 4. 6 mm × 250 mm, 5 μm) with the mobile phase consisting of acetonitrile and water at the flow rate of 0. 8 mL · min-1 , the detection wavelength was set at 250 nm,and column temperature was 25℃.The gradient mobile phase system was used for the fingerprints. Results HPLC fingerprint of Nardostachyos Radix et Rhizoma was established with good chromatography separation and repeatability, which could be used for quality control of Nardostachyos Radix et Rhizoma. After similarity analysis, the results showed that there was no significantly difference between the HPLC fingerprints of samples from different origins,but the content of four major peaks were different.The amount of nardosinone from ten batches of Nardostachyos Radix et Rhizoma was determined by HPLC-DAD, which showed that the contents of nardosinone from Nardostachys jatamansi DC were obviously different with each other. Conclusion The method is sensitive, repeatable and accurate. It can be used as a method of quality control for Nardostachyos Radix et Rhizoma.
