Antibiotic resisitanc and PFGE genotyping of Klebsiella pneumoniae from neonatal ward infections
10.3969/j.issn.1006-5725.2017.20.034
- VernacularTitle:呼吸病房产超广谱β-内酰胺酶肺炎克雷伯菌的耐药性及脉冲场电泳分析
- Author:
Hua FAN
1
;
Li LIU
;
Jingjing ZUO
;
Guangrui BAI
Author Information
1. 161005,黑龙江省齐齐哈尔市第一医院检验科
- Keywords:
exten-spectrumβ-lactamases;
pulse field electrophoresis;
klebsiella pneumonia;
homology
- From:
The Journal of Practical Medicine
2017;33(20):3462-3466
- CountryChina
- Language:Chinese
-
Abstract:
Objective To collecct clinical isolates of Klebsiella pneumonia ,detect the sensitivity of antibiotics,determine the characterization of ESBLs-producing strains,type the isolates by PFGE and investingate the clinical data for analysis. Methods ESBLs-producing strains were tested by inhibitor potentiated disk diffusion rcommended by CLSI2015. The susceptibility to 26 different kinds of antibiotics were testd by CLSI2015 disk diffusion method. The typing of the gene of strains by PFGE was analyzed. Results Sixty ESBLs strains produced in 115 strains of Klebsiellapneumoniae ,which was 100% sensitive to imipenem and meropenem in antimicrobial susceptibility ,and were basically resistant to Amoxacillin ,ampicillin ,piperacillin and the first to the fourth generation of cephalosporins. The 60 ESBLs-producing Klebsiellapneumoniae gene was divided into 14 types (A ~ N)by PFGE,of which type A was the epidemic strain(35%,21/60). Conclusion The incidence rate of ESBLs-producing strains is high in respiratory ward in severe resistant multiantibioticsThe epidemic caused by homologusKlebsiella pneumonia can happen. PFGE is a stable and reliable method in searching the source of noso-comial infecton and is suitable for surveillance in the homologus nosocomial infecton.
