Effect of EZH2 on Hcy-induced cholesterol accumulation of foam cells
10.3969/j.issn.1006-5725.2017.19.006
- VernacularTitle:EZH2在Hcy致泡沫细胞胆固醇聚集中的作用
- Author:
Yinju HAO
1
;
Ning DING
;
Shuqiang LI
;
Lingbo XU
;
Nan LI
;
Yideng JIANG
;
Xiaoling YANG
Author Information
1. 宁夏医科大学药学院药理学系/宁夏医科大学基础医学院心脑血管疾病基础研究重点实验室
- Keywords:
EZH2;
homocysteine;
foam cell;
cholesterol
- From:
The Journal of Practical Medicine
2017;33(19):3176-3180
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of EZH2 on Hcy-induced cholesterol accumulation of foam cells. Methods THP-1 foam cells were divided into control group ,100 μmol/L Hcy group and folic acid group. Lipid droplet in foam cells was tested by Oil red O. TC and TG contents in cells were determined by enzymic meth od. H3K27me3 level and EZH2 protein expression were detected by Western-blot. EZH2 mRNA expression was assayed by q-PCR. H3K27me3 level and TC and TG contents were examined followed by overexpression or knock- down of EZH2. Results After administration of Hcy,TC and TG contents in foam cells were increased (P <0.05). H3K27me3 level and EZH2 expression were also increased(P<0.05). Overexpression of EZH2 caused the expansion of H3K27me3 level,and the TC and TC contents were also increased(P<0.05). Conclusion Regula-tion of H3K27me3 by EZH2 might be involved in Hcy-induced accumulation of cholesterol in foam cells.
