Influence of Alismatis Combined with Bifendateon Cytochrome P450 in Rat
10.3870/j.issn.1004-0781.2017.11.008
- VernacularTitle:泽泻联合联苯双酯对大鼠肝细胞色素P 450酶的影响
- Author:
Yue XU
1
;
Libin WANG
;
Pei LI
;
Yunyun YUAN
;
Wenbin LIU
Author Information
1. 西安医学院药学院
- Keywords:
Alismatis;
Bifendate;
Cytochrome P 450
- From:
Herald of Medicine
2017;36(11):1249-1252
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the influence of Alismatis combined with bifendate on cytochrome P 450 in rat liver microsomes. Methods Twenty four healthy male SD rats were randomly divided into four groups:the experimental groups were given Alismatis at 140 mg·kg-1 , bifendateat 2.18 mg·kg-1 , and Alismatis plus bifendate at 140 mg+2.18 mg·kg-1 ,while the blank control group was given 0. 9% sodium chloride at 5 Ml · kg-1 . The liver microsomes were prepared upon differential centrifugation 7 days after administration.The microsomal protein concentration, cytochrome P450 content, Cytb5 content, NADPH cytochrome C reductase activity and amino pyrine N removal of methyl enzyme activity, erythromycin demethylase activity were determined by UV respectively. Results Compared with the normal control group, the combination use of Alismatis and bifendate redued the microsome content and cytochrome P450 content, while it increased the NADPH cytodrome C activity. The concentrations of cytochrome P450 were both increased by Alismatis and bifendate. Conclusion In contrast, combination ofAlismatis and bifendate reduces cytochrome P450 content which has a negative effect on phase I drug metabolism.Moreover, the combination of Alismatis and bifendate induced NADPH- cytochrome C reductase, accelerated the reduction of cytochrome P450 and inhibited cytochrome P450 isoform CYP2E1 activity.
