Enhancing Radiosensitization Effect of Metformin on Hydrochloride Cervical Cancer Hela Cell Cultured In Vitro
10.3870/j.issn.1004-0781.2017.11.010
- VernacularTitle:盐酸二甲双胍增强宫颈癌细胞对放射线敏感性作用
- Author:
Bing GU
1
;
Fengjun CAO
;
Wei GE
Author Information
1. 武汉大学人民医院肿瘤中心
- Keywords:
Metformin;
Cancer;
cervical;
Radiosensitization
- From:
Herald of Medicine
2017;36(11):1255-1259
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the enhancing radiosensitization effect of metformin on cervical cancer Hela cell and to explore its mechanism. Methods The radiosensitization effect was observed by Clone forming assay;Hela cell cycle distribution was detected by flow cytometry method;Apoptotic changes was measured by fluorescence microscope and DAPI(4', 6-diamidino-2-phenylindole) staining; The expression of vascular endothelial growth factor ( VEGF ) protein was detected by Western blotting. Results Compared with radio alone,the cell survival rate was reduced either by combination with metformin or by metformin alone(P<0.05).The cell survival rate also depended on drug by various schedules and drug concentration(P<0.05).Metformin alone or combined with radiation could arrest cell cycle in G2/M phase(P<0.01),induce cell apoptosis.The levels of protein of VEGF was decreased by metformin, radiation alone or combination( P<0.05) . Conclusion Metformin has a radiosensitization effect on Hela cell cultured in vitro, which may be related to arresting in G2/M phase, inducing cell apoptosis and down-regulating the level of VEGF protein.
