Effects of Bcl-2 and BaK gene silencing on cell apoptosis and osteogenesis activity of MG-63 cell lines
10.3969/j.issn.1006-5725.2017.17.002
- VernacularTitle:Bcl-2和BaK基因沉默对MG-63细胞凋亡和成骨活动的影响
- Author:
Xianwen YANG
1
;
Hongxing HUANG
;
Lei WAN
;
Hong HUANG
;
Fan WANG
;
Shuang CHAI
Author Information
1. 510006,广州中医药大学第三临床医学院
- Keywords:
Osteoporosis;
Bcl-2;
BaK;
cell apoptosis
- From:
The Journal of Practical Medicine
2017;33(17):2813-2816
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Bcl-2 and BaK gene silencing on cell apoptosis ,osteogen-esis activity and free Ca2+concentration of MG-63 cell lines. Methods The siRNA sequences targeted Bcl-2 and BaK respectively were designed;Bcl-2 and BaK silencing adenovirus vector scramble RNA vector and empty vec-tor were constructed to transfect MG-63 cell lines. MTT method was used to examine cell viability;ALP and flow cytometry were conducted to observe osteogenesis activity and free Ca2+concentration. Results Bcl-2 gene silenc-ing decreased cell viability,reduced osteogenesis activity and increased free Ca2+ concentration when compared with controls but BaK gene silencing had the opposite effects. The effect of Bcl-2+BaK gene silencing on cell was similar to the empty control. Conclusions Cell apoptosis,osteogenesis activity and free Ca2+concentration of MG-63 change following Bcl-2 and BaK gene silencing,implicating their roles in osteoporosis.
