Overexpression of miRNA-199a-3p targets at expression of MAP3K4 in gastric cancer
10.3969/j.issn.1006-5725.2017.17.003
- VernacularTitle:miRNA-199a-3p调控MAP3K4在胃癌中的表达
- Author:
Xiaochun MIN
1
;
Tingting WU
;
Ji ZENG
;
Xiaoxue ZHANG
;
Xiaoming LIU
;
Xiaopeng JING
;
Ce JIN
Author Information
1. 华中科技大学同济医学院附属普爱医院检验科
- Keywords:
Stomach neoplosms;
miR-199a-3p;
MAP3K4
- From:
The Journal of Practical Medicine
2017;33(17):2817-2820
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miRNA-199a-3p overexpression on the expression of MAP3K4 protein in gastric cancer. Methods 35 gastric cancers and the matched adjacent tissue specimens were collected. Expression of miRNA-199a-3p and MAP3K4 were detected by stem-loop real-time reverse transcription polymerase chain reaction and western blot. Cell transfection was employed to explore the regulation of miRNA-199a-3p on MAP3K4 gene. Luciferase reporter assay was performed to identify target MAP3K4 gene. Results Com-pared with the adjacent tissue specimens ,miRNA-199a-3p was upregulated in the gastric cancers ,and MAP3K4 protein was down-regulated in the gastric cancers. Cells transfected with miR-199a-3p mimics showed lower MAP3K4 protein. MAP3K4 was identified as target gene of miR-199a-3p. Conclusions miRNA-199a-3p acts as an oncogene in gastric cancer and functions by targeting MAP3K4.
