Protective effect of tanshinoneⅡA on radiation injury of cochlea
10.3969/j.issn.1006-5725.2017.17.004
- VernacularTitle:丹参酮ⅡA对放射性内耳损伤的保护作用
- Author:
Peixin TAN
1
;
Shasha DU
;
Chen REN
Author Information
1. 广东省人民医院放疗科
- Keywords:
radiation injury;
ototoxicity;
auditory brainstem response;
distortion product otoacous-tic emissions;
tanshinone
- From:
The Journal of Practical Medicine
2017;33(17):2821-2826
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effects on radiation injury of guinea pigs 'cochlea by sul-fotanshinoneⅡA sodium injection. Methods 20 guinea pigs(40 ears)were randomly divided into control group , radiotherapy(RT)group,radiotherapy and drug(RT+D)group and drug(D)group. The guinea pigs in the RT+D group and the D group were injected intraperitoneally with tanshinone on 2 days before the irradiation and last-ed for 7 days,while the guinea pigs in the control group and the RT group were injected intrapertoneally with equal amount of physiological saline at the same time. All the guinea pigs underwent audiologic test with DPOAE and ABR at different time points before and after the irradiation,which were d0,d7 and d8w. All pigs were sacrificed after the completion of the audiologic experimental and for histologic observation. Results After the irradiation, the DPOAE amplitudes of the guinea pigs in both RT group(F=1134.064,P<0.001)and RT+D group(F=664.185,P < 0.001)decreased significantly,but the range in the RT + D group was lower than in the RT group (P<0.001). The ABR threshold of the guinea pigs raised significantly in the RT group(F=12.228,P=0.002 <0.05),but not in the RT+D group(F=2.867,P=0.102>0.05). Histological examination showed that the dam-age degree of hair cells,stria vascularis and the spiral ganglion cells in the organ of Corti of the guinea pigs were lower than in the RT + D group,but higher in the RT group. Conclusion Sulfotanshinone ⅡA sodium injection can provide protection against radiation injury of guinea cochlea.
