Expression of SDF-1 in obstructive jaundice rat various tissues
10.3969/j.issn.1006-5725.2017.17.005
- VernacularTitle:SDF-1在梗阻性黄疸大鼠不同组织中的表达
- Author:
Zhen YANG
1
;
Jiaolin NING
;
Jianteng GU
;
Bin YI
;
Kaizhi LU
Author Information
1. 第三军医大学第一附属医院麻醉科
- Keywords:
obstructive jaundice;
liver cirrhosis;
stromal cell derived factor-1;
hepatopulmonary syndrome
- From:
The Journal of Practical Medicine
2017;33(17):2826-2829
- CountryChina
- Language:Chinese
-
Abstract:
Objective To dynamically observe the SDF-1 level in plasma,bone marrow,liver,lung and kidney,and to investigate their significance in obstructive jaundice and its complications. Method 48 male SD rats weighing about 200g were randomly divided into Sham group and CBDL group. The serum ALT ,AST and se-rum total bilirubin(TBIL)were detected at 7 d,14 d and 21 d after operation. General condition of rats in the two groups were observed. ELISA was applied in detecting expression of SDF-1 in plasma and supernate of tissue ho-mogenate. And mRNA expression of SDF-1 at different time was detected by qPCR assay. Results ALT,AST,TB increased rapidly after CBDL operation,the difference was significant compared with Sham group(P <0.05). The SDF-1 expression of plasma,liver tissue,lung tissue in CBDL rats at different time points were significantly higher than in Sham group. No significant difference was found in renal tissue. SDF-1 expression of bone marrow in 7 d,14 d,21 d was significantly lower in CBDL group than in Sham group. Conclusion Expression of SDF-1 in liver and lung tissues of obstructive jaundice rats significantly increased ,and decreased in marrow bone. This change may promote related stem cells mobilization and contribute to the pathological changes of obstructive jaun-dice.
