Determination of Fluvoxamine in Human Plasma by FPLC-MS/MS
- VernacularTitle:快速液相色谱联用质谱法测定人血浆氟伏沙明的药物浓度
- Author:
Jie LI
1
;
Yi YANG
;
Jialong YE
;
Sanlan WU
Author Information
1. 华中科技大学同济医学院附属协和医院药剂科 武汉430022
- Keywords:
Fluvoxamine;
Drug concentration in blood;
FPLC-MS/MS
- From:
China Pharmacist
2017;20(10):1728-1730
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an FPLC-MS/MS method for the determination of fluvoxamine in human plasma. Methods:The separation was performed on an Inertsil? ODS-SP column(2. 1 × 100 mm, 3 μm). The mobile phase was acetonitrile-2 mmol· L-1 ammonium acetate (45: 55, v/v) containing 0. 1% formic acid at a flow rate of 0. 3 ml·min-1 . Lansoprazole was used as the internal standard( IS) . Electrospray ionization ( ESI) source was applied and operated in a positive ion model. Multiple reaction moni-toring (MRM) model with the transitions of fluvoxamine m/z 319. 1→m/z 69. 8 and lansoprazole m/z 370. 2→m/z 252. 1 was used to quantify fluvoxamine and IS, respectively. Results:In human plasma, the standard curve was linear within the range of 1-100 μg· L-1 . The lower limit of quantification of fluvoxamine( LLOQ) was 1μg·L-1 . The intra-day RSD was less than 5%, the inter-day RSD was less than 10%, and the method recovery was 85%-95%. Conclusion:The method is simple, sensitive, accurate and reproduci-ble. It is applicable in the pharmacokinetic study of fluvoxamine for clinical pharmacokinetics and bioequivalence studies.
