Content Determination of Polyphyllin Ⅰ and Ⅱ in Kangkeling Mixture by HPLC
10.3969/j.issn.1005-5304.2017.11.018
- VernacularTitle:康咳灵合剂中重楼皂苷Ⅰ、Ⅱ含量测定
- Author:
Min PENG
1
;
qin Mei SHI
;
dong Xiao TONG
;
ying Shui YANG
;
Yan XU
Author Information
1. 南通市中医院
- Keywords:
Kangkeling Mixture;
HPLC;
polyphyllinⅠ;
polyphyllinⅡ
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(11):75-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determining the contents of polyphyllin Ⅰ and polyphyllin Ⅱ in Kangkeling Mixture. Methods The contents of polyphyllin Ⅰ and Ⅱ were determined by HPLC gradient elution. Poroshell 120 Ec-C18 column (4.6 mm × 150 mm, 4 μm) was used; Acetonitrile-water (A:B) was set as the mobile phase; the flow rate was 1.0 mL/min; the detection wavelength was 210 nm; column temperature was 30 ℃. Results Polyphyllin Ⅰ showed good linear relationship in the range of 1.009–10.09 μg (r=0.999 6), and the average recovery was 97.31% (RSD=2.05%, n=6). Polyphyllin Ⅱ showed good linear relationship in the range of 0.640 5–6.405 μg (r=0.999 8), and the average recovery was 96.41% (RSD=1.67%, n=6). Conclusion The method is simple, with good repeatability and accurate results, which can be used to determine the contents of polyphyllin Ⅰ and Ⅱ in Kangkeling Mixture.
