Study on the effect of single dose intraosseous injection of simvastatin on tumor vascular normalization in murine breast cancer
10.3969.j.issn.1671-7856.2017.010.001
- VernacularTitle:骨内单次注射辛伐他汀促进小鼠乳腺癌肿瘤血管正常化的研究
- Author:
Bao HAI
1
;
Can LIU
;
Wen ZHANG
;
xiong Jun ZHU
;
shan Bao CAO
;
li Chun SONG
Author Information
1. 北京大学第三医院骨科
- Keywords:
Simvastatin;
Intraosseous injection;
Vasculature normalization;
Breast cancer;
Pericyte
- From:
Chinese Journal of Comparative Medicine
2017;27(10):1-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of single dose intraosseous injection of simvastatin on tumor vascular structure and function in murine breast cancer. Methods BALB/c mice and 4T1 murine breast cancer cells were used to establish a subcutaneous xenograft model. The mouse model of orthotopic breast cancer received intraosseous injection of a single dose of simvastatin (50 μg) or vehicle only. Frozen tumor tissue sections were prepared for co-immunostained with CD31 andα-SMA. Evans blue dye was injected into the tail vein to observe the vascular permeability. The expression level of HIF-1αwas detected by immunohistochemistry. Results Immunofluorescence dual staining showed that intraosseous injection of simvastatin increased the number of perivascular pericytes in the tumor vessel(P < 0. 05), Evans blue dye content showed that in vivo vessel permeability in the tumor tissue was significantly decreased(P < 0. 05), and the immunohistochemistry results showed that local hypoxic area was significantly improved. Conclusions Single dose intraosseous injection of simvastatin can promote the normalization of tumor vasculature by improving the coverage of pericytes.
