Differential proteomic analysis of liver tissues between male and female C57BL / 6J mice by 2D-DIGE
10.3969.j.issn.1671-7856.2017.10.004
- VernacularTitle:荧光双向电泳检测雌雄小鼠肝组织蛋白组学的差异
- Author:
na Zhuo RONG
1
;
ling Hui LI
;
hui Peng DONG
;
ting Ting FAN
;
Juan LI
;
Yi ZHAO
;
jin Fu WANG
;
guo Ai WANG
;
yu Jing WANG
Author Information
1. 大连医科大学实验动物中心
- Keywords:
Liver;
Gender difference;
Proteomic;
Bioinformatics;
Two dimension difference gel electrophoresis;
2D-DIGE
- From:
Chinese Journal of Comparative Medicine
2017;27(10):16-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the differential proteomic expressions between the liver tissues of male and female mice, and investigate the mechanisms underlying gender differences in liver diseases. Methods Two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry ( MALDI-TOF-MS) were used to identify the differentially expressed proteins in the liver tissues of male and female C57BL/6J mice. The differentially expressed proteins were validated by Western blot and further analyzed by bioinformatics, including Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG). Results Among the auto-detected 1767 protein spots by 2D-DIGE, 325 protein spots were differentially expressed (|ratio|≥1. 5, P< 0. 05) between the liver tissues of male and female mice, in which 78 spots were randomly selected for MALDI-TOF-MS identification and finally 48 distinct proteins were obtained. Compared with females, 14 and 34 proteins were up-or down-regulated in males, respectively. Among them, 6 differentially expressed proteins were validated by Western blot which confirmed the reliability of 2D-DIGE results. GO analysis showed that the differentially expressed proteins in the liver tissues of male and female mice are associated to various cellular component, molecular function and biological process. 6 pathways were significantly different between the liver tissues of males and females depending on KEGG analysis. Conclusions The proteomic data and related analysis of the liver tissues of C57BL/6J mice offer crucial clues for elucidating the underlying mechanisms of different gender effects on liver diseases.
