Effect of Mac-1 deficiency on the tumor growth of melanoma in mice
10.3969.j.issn.1671-7856.2017.10.005
- VernacularTitle:Mac-1基因缺失对黑色素瘤生长的影响
- Author:
Jian CHEN
1
;
fa You DUAN
;
jin Zhi YE
;
jing Li WANG
;
qian Qian ZHANG
Author Information
1. 广东药科大学基础学院
- Keywords:
Mac-1;
Melanoma;
Tumor growth;
Mice
- From:
Chinese Journal of Comparative Medicine
2017;27(10):23-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of Mac-1 deficiency on tumor growth of melanoma. Methods The population of Mac-1 gene knock-out ( Mac-1 -/ -) mice was expanded. B16-F10 cells were subcutaneously injected into the C57BL/6J mice (control group) and Mac-1 -/ -mice (experiment group), respectively. Subsequently,the survival rate, tumor volume and body weight were recorded. The proliferation and infiltration of macrophages were detected by immunohistochemistry. Results The survival rate of Mac-1 -/ - mice was significantly improved compared with the C57BL/6J mice (P ﹤0. 001). The tumor volume and body weight were remarkably decreased in the Mac-1 -/ - mice compared with the control group (P﹤0. 001). Meanwhile, the tumor cell proliferation index was decreased in the Mac-1 -/ - mice compared with the control group (P﹤0. 01). Furthermore, the infiltration of macrophages in the tumor tissues was also decreased in Mac-1 -/ - tumor mice compared with control group. Conclusions Mac-1 gene deletion can significantly suppress melanoma growth.