1α,25( OH) 2 D3 regulates expression of lubricin of chondrocytes in rat articular cartilage
10.3969.j.issn.1671-7856.2017.010.006
- VernacularTitle:1α,25(OH)2 D3对大鼠关节软骨细胞润滑素表达的影响
- Author:
dong Guo NIU
1
;
wei Si LI
;
jun Zhong LIU
;
li Chun SONG
;
jie Hui LENG
Author Information
1. 北京大学第三医院骨科
- Keywords:
Vitamin D;
Lubricin;
Chondrocytes;
Osteoarthritis
- From:
Chinese Journal of Comparative Medicine
2017;27(10):28-33,39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of vitamin D on synthesis and secretion of lubricin in chondrocytes at the cellular level. Methods Rat articular chondrocytes were stimulated by TNF-α. Normal and inflammatory chondrocytes were treated by different doses of vitamin D respectively. ELISA and Western Blot were used to detect the secretion of lubricin in the supernatant and the synthesis level in the cells. Results TNF-α significantly reduced the activity of both normal chondrocytes and chondrocytes in inflammatory state. TNF-α also significantly reduced the expression of lubricin in the cells and supernatant. 1α,25(OH)2D3 increased the activity of both normal chondrocytes and chondrocytes in inflammatory state. 1α,25(OH)2D3 significantly elevated the secretion and expression of supernatant and intracellular lubricin only in chondrocytes stimulated by TNF-α in a dose-dependent manner, but not in normal chondrocytes. Conclusions Vitamin D can promote the secretion and expression of lubricin in inflammatory state chondrocytes, which may act as one of the mechanisms of vitamin D protecting the cartilage surface in osteoarthritis.
