Investigation of postnatal stem cells from canine dental tissue and bone marrow.
10.5051/jkape.2009.39.2.119
- Author:
Min Ju JHIN
1
;
Young Sung KIM
;
Su Hwan KIM
;
Kyoung Hwa KIM
;
Chul Woo LEE
;
Ki Tae KOO
;
Tae Il KIM
;
Yang Jo SEOL
;
Young KU
;
In Chul RHYU
;
Chong Pyoung CHUNG
;
Yong Moo LEE
Author Information
1. Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University, Korea. lee@snu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
stem cells;
differentiation;
periodontal ligament;
regeneration
- MeSH:
Adipocytes;
Animals;
Antigens, Surface;
Bone Marrow;
Calcium Phosphates;
Cell Differentiation;
Chondrocytes;
Durapatite;
Mesenchymal Stromal Cells;
Mice;
Mice, Nude;
Osteoblasts;
Osteogenesis;
Periodontal Ligament;
Regeneration;
Stem Cells;
Transplants
- From:The Journal of the Korean Academy of Periodontology
2009;39(2):119-128
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The aim of this study was to evaluate the stemness of cells from canine dental tissues and bone marrow. METHODS:Canine periodontal ligament stem cells (PDLSC), alveolar bone stem cells (ABSC) and bone marrow stem cells(BMSC) were isolated and cultured. Cell differentiations (osteogenic, adipogenic and chondrogenic) and surface antigens (CD146, STRO-1, CD44, CD90, CD45, CD34) were evaluated in vitro. The cells were transplanted into the subcutaneous space of nude mice to assess capacity for ectopic bone formation at 8 weeks after implantation. RESULTS: PDLSC, ABSC and BMSC differentiated into osteoblasts, adipocytes and chondrocytes under defined condition. The cells expressed the mesenchymal stem cell markers differently. When transplanted into athymic nude mice, these three kinds of cells with hydroxyapatite /beta tricalcium phosphate (HA/TCP) carrier showed ectopic bone formation. CONCLUSIONS: This study demonstrated that canine dental stem cells have stemness like bone marrow stem cells. Transplantation of these cells might be used as a therapeutic approach for dental stem cell-mediated periodontal tissue regeneration.
