Progress in application of affinity chromatography inpharmaceutical analysis
10.7652/jdyxb201706001
- VernacularTitle:亲和色谱技术在药物分析中的应用进展
- Author:
cen Si WANG
1
;
shuang Xiao HE
Author Information
1. 西安交通大学药学院
- Keywords:
affinity chromatography;
pharmaceutical analysis;
chiral drug separation;
active ingredient screening;
drug-protein interaction;
biomacromolecule
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(6):777-784
- CountryChina
- Language:Chinese
-
Abstract:
Affinity chromatography (AC)is a type of liquid chromatography that makes use of biological-like interactions for separation and specific analysis of bioactive components. It has been widely used as a high-throughput screening method for the separation,screening and purification of the target molecules from complex samples with advantages such as high selectivity and high recovery efficiency.This article summarizes the biological effects of affinity chromatography, molecular imprinting chromatography, and dye ligands affinity chromatography.The review also encompasses the application of AC in the separation of chiral drugs,screening of active components,purification of target protein,and mechanism of the drug-protein interaction.Moreover,the prospects of its applications are also discussed.
