Localization of transplanted menstrual-derived stem cells in premature ovarian failure model of mice
10.7652/jdyxb201706005
- VernacularTitle:经血来源干细胞移植在小鼠卵巢早衰模型中的定位分布
- Author:
Zhen WANG
1
;
rong Kang HUANG
;
ling Yue WANG
;
Wei WANG
;
yan Hai BAI
;
yuan Xin YANG
Author Information
1. 西安交通大学第一附属医院妇产科
- Keywords:
menstrual-derived stem cell;
stem cell transplantation;
premature ovarian failure
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(6):803-808
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the localization of transplanted human menstrual-derived stem cells (MenSCs)in the premature ovarian failure (POF)mouse model.Methods Mice were intraperitoneally injected with cisplatin to create the POF mouse model.Ninety mice aged 8 weeks were randomly divided into MenSCs transplantation group,POF model group and blank control group.Green fluorescence protein (GFP)-expressing MenSCs were transplanted to MenSCs transplantation group by the tail vein;meanwhile the same volume of PBS was injected into the mice in POF model group and blank control group.Physiological characteristics of mice,serum concentration of FSH and E2,ovarian histology and distribution of green fluorescence signal in the ovarian tissues were assessed in the three groups at 10 days after transplantation.Results Following anorexia and lethargy,the average weight of mice in POF mouse model decreased compared with that in blank control group. The concentration of FSH increased but E2 level decreased significantly in POF model group.Meanwhile,the number of primordial,growing and mature follicles reduced significantly and interstitial fibrosis was observed in the ovary ofPOF model group.After MenSCs transplantation,the average weight of mice in MenSCs transplantation group increased gradually with the improvement of feeding and activities.Ten days after injection of MenSCs,sex hormone levels and the number of follicles returned to normal values.The level of E2 was significantly higher and the level of FSH was lower,while the number of primordial,growing as well as mature follicles in MenSCs transplantation group was significantly higher than that in POF model group.By observing the location of GFP-MenSCs,we found that GFP-positive cells were located in the interstitium but not in follicles,granulose cells,the liver,the kidney,or lung tissue.Conclusion MenSCs can home to the ovary and be located in the interstitium in the POF mouse model.MenSCs can repair ovarian injury and improve endocrine functions of the ovary.
