Effect of hepatitis B virus X on the apoptosis of placental trophoblastic cells and its potential mechanism
10.7652/jdyxb201706006
- VernacularTitle:HBxAg对胎盘滋养细胞凋亡的影响及其作用机制
- Author:
min Wei WANG
1
;
xing Yong YUAN
;
Chen LI
;
xia Yi PAN
;
Na XU
;
qin Gui BAI
Author Information
1. 西安交通大学第一附属医院妇产科
- Keywords:
JEG-3;
HTR-8;
hepatitis B virus X;
transient transfection;
apoptosis;
human placental tropho-blastic cell
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(6):809-814
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hepatitis B virus X (HBx)protein on the apoptosis of placental trophoblastic cells and its potential mechanism.Methods A pcDNA3.1 expression vector of HBx gene was constructed and transfected into JEG-3 and HTR-8 human placental trophoblastic cell lines,respectively.After transfection for 48 h,RT-PCR and immunofluorescence analyses were made to detect HBx mRNA and protein expressions.Flow cytometry was used to detect the early apoptosis status of JEG-3 and HTR-8 cells.The expressions of PI3K and p-Akt were detected by immunofluorescence and Western blotting.Results After transfection for 48 h,RT-PCR and immunofluorescence analyses showed that HBx mRNA and protein expressions were detected in JEG-3 and HTR-8 cells.Flow cytometry revealed that early apoptosis of JEG-3 and HTR-8 cells was reduced by pcDNA-HBx transfection (P <0.05).Immunofluorescence and Western blotting showed that PI3K and p-Akt were significantly upregulated in HTR-8 cells (P < 0.05 ).Conclusion HBx gene can be transfected into JEG-3 and HTR-8 human placental trophoblastic cell lines,respectively.After the transfection,the early apoptosis of JEG-3 and HTR-8 cells is reduced.Its inhibition on apoptosis is related to the activation of the PI3K/Akt signaling path-way.
