Relationship between expression of CD142 protein and the promoter methylation in the placenta in severe preeclampsia
10.7652/jdyxb201706019
- VernacularTitle:重度子痫前期胎盘中CD142的表达与其启动子区甲基化程度的关系
- Author:
Rui LIU
1
;
Qiang MA
;
xin An WEN
;
yan Gai TIAN
;
juan Wen WANG
;
Sa LIN
;
min Wei WANG
Author Information
1. 西安交通大学第一附属医院妇产科
- Keywords:
severe preeclampsia;
CD142;
promoter;
methylation;
placenta
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(6):883-887
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between expression of CD142 protein and the promoter methylation in the placenta of severe preeclampsia patients.Methods We assessed 24 patients complicated with severe preeclampsia as case group and 24 normal pregnant women as control group via qRT-PCR, immunohistochemistry and Western blotting for CD142 expression and MSP technology for methylation in CD142 promoter region.Results The relative expression quantity of CD142 mRNA in severe preeclampsia group (1.45± 0.42)was higher than that in normal group (0.25±0.28)(P <0.05).The expression quantity of CD142 protein in severe preeclampsia group (0.857±0.043)was higher than that in normal group (0.248 ±0.035)(P <0.05).The positive rate of CD142 promoter region methylation was lower in severe preeclampsia group than in normal group (29.2% vs .100.0%,χ2 =36.11,P <0.001)while the positive rate of CD142 promoter region unmethylation was higher than that in the latter group (100.0% vs .20.8%,χ2 =29.85,P <0.001).A negative correlation was found between the CD142 promoter methylation level and the expression quantity of CD142 protein (r = -0.909,P <0.05).Conclusion The expression of CD142 protein regulated by the promoter methylation plays a crucial role in the mechanism of severe preeclampsia.
