Phenotypic characterization of PMA-induced THP-1 macrophages analyzed by flow cytometry
10.3969.j.issn.1671-7856.2017.10.003
- VernacularTitle:流式细胞术分析PMA诱导THP-1分化为巨噬细胞的表型特征
- Author:
ying Zhuo PENG
1
;
Xiang LI
;
Zhe CONG
;
Jing XUE
Author Information
1. 北京协和医学院比较医学中心
- Keywords:
THP-1;
Macrophage;
Phorbol 12-myristate 13-acetate;
PMA;
Flow cytometry
- From:
Chinese Journal of Comparative Medicine
2017;27(10):10-15,22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the characteristics of the subtype of PMA-induced THP-1 macrophages by flow cytometry analysis. Methods THP-1 monocytic cells differentiate into macrophages promoted by PMA, then induced into M1 and M2 by adding different cytokines, such as LPS,IL-6 and IFN-γ for THP-1-M1, IL-4,IL-13 and IL-6 for THP-1-M2. Morphology of cells were observed under a microscope and the expression of CD14, CD68, CD16, CD80, CD86, CD163, CD206, CD209, CD83, CD1a, CD11c, HLA-DR were detected by flow cytometry. Results The macrophages stimulated by PMA became adherent;THP-1-M1 and THP-1-M2 lost their spherical morphology, appeared more irregular with many obvious projections. The expression of CD83, CD1a, CD11c, HLA-DR which had the function of antigen presenting on the surface of THP-1-Mφwere very low, and most of them were negative, but those of THP-1-M1 and THP-1-M2 were very high. Conclusions The macrophages differentiated from THP-1 stimulated by PMA are weak in antigen presenting function.
