Surveillance and analysis of virus antibodies in conventional rabbits and guinea pigs in Guangdong Province during 2014-2016
10.3969.j.issn.1671-7856.2017.10.013
- VernacularTitle:广东省2014~2016年普通级兔和豚鼠病毒抗体监测与分析
- Author:
ke Rui WU
1
;
Jing WANG
;
chun Jin PAN
;
zhen Xiu LI
;
ling Mei CHEN
;
wu Shu HUANG
;
gui Fan MIN
Author Information
1. 广东省实验动物监测所
- Keywords:
Conventional animals;
Rabbits;
Guinea pigs;
Virus;
Antibody detection
- From:
Chinese Journal of Comparative Medicine
2017;27(10):65-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the possibilities of screening SPF rabbits and guinea pigs from conventional animals, viral antibodies of the conventional rabbits and guinea pigs bred by licensed companies in Guangdong province during 2014-2016 were determined according to the standard of SPF animals in GB14922. 2. Methods Nine batches of 167 rabbit sera and 155 guinea pig sera were sampled from 6 companies. Serum antibodies to virus were determined by ELISA according to GB14922. 2. Results Positivity of antibody to rabbit hemorrhagic disease virus (RHDV) was 82. 2%(129/157) for the vaccinated rabbits, and negative result were obtained for unvaccinated rabbits. Positive rate of rabbit rotavirus (RRV) was 42. 5% (71/167). No positive antibody responses to Sendai virus were detected out in all rabbits. The positive rates of guinea pig reovirus type III (REO-3) and pneumonia virus of mice (PVM) were 52. 9%(82/155)and 20% (31/155) respectively. Antibody responses to Sendai virus ( SV) and lymphocytic choriomeningitis virus ( LCMV) were negative in all guinea pigs. Conclusions Although the conventional rabbits and guinea pigs could meet the national standard, higher infection rates of virus excluded that SPF animals emerged in conventional animals, indicating that selection of SPF animals from conventional colonies is impracticable.
