Simultaneous Detection of Fat-soluble Vitamins in Health Foods by UPLC-MS/MS and Its Application
- VernacularTitle:液相串联质谱法测定保健食品中脂溶性维生素方法建立及其应用
- Author:
Xiao NING
1
;
Wenchao GAO
;
Jiaoyi LUO
;
Jin CAO
;
Hong DING
Author Information
1. 中国食品药品检定研究院 北京100050
- Keywords:
Health food;
Ultra-performance liquid chromatography-tandem mass spectrometry ( UPLC-MS/MS);
Fat-soluble vita-mins
- From:
China Pharmacist
2017;20(10):1768-1772,1780
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an ultra-performance liquid chromatography-tandem mass spectrometry ( UPLC - MS/MS ) method for the determination of nine fat-soluble vitamins in health foods. Methods:The samples were extracted by ultrasonic in organic solvents followed by the separation on a Waters ACQUITY UPLC BEH C18 column(100 mm × 2. 1 mm, 1. 7 μm) with gradient elution of 0. 1% formic acid in methanol and 0. 1% formic acid solution as the mobile phase and in a mode of atmosphere pressure chemical i-onization multiple reaction monitoring (MRM). Results:The method exhibited a good linear relationship (r≥0. 99) with the detection limit between 0. 9 and 38. 3μg/100 g for the nine fat-soluble vitamins. The recoveries were 78. 5%-114. 9% and the RSDs were 1. 57%-11. 1%(n=6). Conclusion:The analytical method is simple, rapid, accurate and sensitive, and suitable for the determina-tion of fat-soluble vitamins in nutritional supplements.
