The neuroprotective machanism of Delta-opioid receptor activator against global cerebral ischemia-reperfusion
10.3969/j.issn.1002-0152.2017.08.001
- VernacularTitle:δ阿片受体激动剂在全脑缺血再灌注损伤中的神经保护机制
- Author:
Jieyun HAN
1
;
Qing LIANG
;
Xiang PENG
;
Weiqing HUANG
;
Zijing LIANG
Author Information
1. 广州医科大学附属第一医院急诊科 广州510120
- Keywords:
Receptor;
Opioid;
Delta;
Reperfusion injury;
Apotosis
- From:
Chinese Journal of Nervous and Mental Diseases
2017;43(8):449-452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Delta-opioid receptor activator DADLE on the expression of the p38 MAPK and the neuronal apoptosis after global cerebral ischemia-reperfusion and explore the neuroprotective mechanisms of DADLE.Methods Global cerebral ischmemia-reperfusion models in rats were induced by bilateral common carotid artery occlusion combined with hypotension.Rats were randomly divided into sham group (n=10),ischemia-reperfusion group(n=10) and three treatment groups with different doses of DADLE (2 mg group,3 mg group,5 mg group,n=10).TUNEL method and Western blot analysis were used to measure apoptotic neurons and expression levels of p38 MAPK phosphorylation,repectively.Results DADLE treatment significantly reduced neuron apoptosis (P＜0.05).The expression levels of p38 MAPK were increased in ischemia-reperfusion group than in sham group (P＜ 0.05).In DADLE treated groups,the expression levels of p38 MAPK were dose-dependently decreased compared with the ischemia-reperfusion group.Conclusion Delta-opioid receptor activator DADLE can be neuroprotective against global I/R injury.Attenuation of apoptosis and p38 MAPK signal pathway might be involved in the neuroprotective mechanism of DADLE.
