The role of aptamer-siRNA nucleic acid compound in promoting the apoptosis of K562 cells
10.7652/jdyxb201706015
- VernacularTitle:aptamer-siRNA核酸复合物对K562细胞凋亡的促进作用
- Author:
hua Jian LU
1
;
qiong Zhao CUI
;
xia Zhao ZHAO
;
Ying LÜ
;
Yi XU
Author Information
1. 石家庄市第五医院检验科
- Keywords:
chronic myeloid leukemia (CML);
Aptamer-siRNA compound;
K562 cell;
apoptosis
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(6):862-865,871
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of aptamer-siRNA nucleic acid compound on the apoptosis of K562 cells in human chronic myelogenous leukemia (CML)and explore its acting mechanisms.Methods K562 cells were transfected with different concentrations of aptamer-siRNA solution.The effects of aptamer-siRNA on the proliferation and apoptosis of K562 cells were detected by MTT method and AnnexinV/PI double staining method,respectively.The effects of aptamer-siRNA on the expressions of bcl-2,Bax and casepase-3 at protein and mRNA levels in K562 cells were detected by Western blot and RT-PCR method,respectively.Results Compared with the control group,the proliferation of K562 cells was significantly inhibited,early apoptosis rate of K562 cells increased significantly,the expression levels of bcl-2 protein and mRNA were significantly decreased,while the expression levels of Bax and caspase-3 protein and mRNA were significantly increased after transfection with aptamer-siRNA (P <0.05).Aptamer-siRNA nucleic acid complex at the concentration of 50 -250 μmol/L)had a significant dose-effect relationship on bcl-2,Bax and caspase-3 mRNA.Conclusion Aptamer-siRNA nucleic acid compound can promote the decreased number of bcl-2 gene and the growth of Bax and caspase-3 genes,thus promoting the apoptosis of K562 cells.
