Dauricine enhances the sensitivity of 5-fluorouracil in human breast cancer MCF-7 cells
10.11904/j.issn.1002-3070.2017.05.001
- VernacularTitle:蝙蝠葛碱在人乳腺癌MCF-7细胞对5氟尿嘧啶敏感性的研究
- Author:
Hongyang LI
1
;
Liang SUN
;
Xing JIANG
;
Guangjun YUAN
;
Yan LIU
Author Information
1. 哈尔滨医科大学附属第四医院肿瘤内科
- Keywords:
Dauricine;
5-Fluorouracil;
Breast cancer;
MCF-7 cells
- From:Practical Oncology Journal
2017;31(5):385-389
- CountryChina
- Language:Chinese
-
Abstract:
Objective The objective of this study was to investigate the effect of dauricine on the sensi-tivity of 5-fluorouracil(5-FU)in human breast cancer MCF-7 cells.Methods MCF-7 cells were treated with 2.5 μg/mL of dauricine,50 μg/mL of 5-FU,or 2.5 μg/mL of dauricine with 50 μg/mL of 5-FU,the cell proliferation was measured by MTT assay.The cell migration was determined by Transwell assay;The cell apopto-sis was detected by DAPI staining;The expression of cyclin D1 and Bcl-2 gene was examined by Western blot. Results The results showed that the combination of subthreshold concentration of dauricine enhanced the inhibi-tory effect of 5-FU on proliferation in MCF-7 cells.The combined use of subcutaneous concentration of dau-ricine further aggravated the inhibitory effect of 5-FU on cell migration.The combination of subcapsular dau-ricine enhanced the induction of apoptosis by 5-FU.The combination of dauricine with 5-FU could inhibit the expression of cyclin D1 and Bcl-2 protein in MCF-7 cells.Conclusion Dauricine can effectively enhance the sensitivity of 5-FU in human breast cancer MCF-7 cells.
