Metabolomic analysis of the changes of pathways in immunosuppressive mechanism caused by cyclophosphamide in mice
10.3969/j.issn.1005-4847.2017.05.013
- VernacularTitle:环磷酰胺对小鼠免疫抑制作用过程中代谢通路变化的代谢组学研究
- Author:
Xuan SHI
1
;
Yong YANG
;
han Wei QIN
;
hong Jia XU
;
jian Da YANG
;
lei Yan GUO
Author Information
1. 重庆市中药研究院
- Keywords:
Cyclophosphamide;
Immunosuppression;
Metabolomics;
Metabolic pathway
- From:
Acta Laboratorium Animalis Scientia Sinica
2017;25(5):539-543
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of metabolic pathways in the process of immunesuppression in mice caused by cyclophosphamide by metabolomic analysis. Methods Flow cytometry was used to detect the changes of immune cells, and the results were analyzed using partial least squares model. The potential biomarkers were screened by variable importance in projection ( VIP) parameters in the partial least squares model, and the differential metabolites were determined by statistical analysis. The differential metabolites were identified using the metabolomics rapid identification and analysis software. Nine kinds of metabolites were identified by searching and calculation, and result of pathway enrich-ment showed three differential metabolic pathways. Results Cyclophosphamide had a significant effect on the biosynthesis of unsaturated fatty acids, metabolism of mitochondrial fatty acids, metabolism of glycophospholipid, biosynthesis of steroid hormones, metabolism of arachidonic acid, metabolism of fatty acids, and the biosynthesis of pyrimidine. Conclusions The most important metabolic pathways affected by cyclophosphamide in the normal body are the metabolism of arachidonic acid, glycophospholipid and pyrimidine.
