Establishment and application of an in vitro mouse intestinal organoid culture system
10.3969/j.issn.1005-4847.2017.05.008
- VernacularTitle:小鼠小肠类器官体外培养体系的建立与应用
- Author:
sheng Guang WAN
1
;
yan Xin ZHANG
Author Information
1. 上海中医药大学附属普陀医院
- Keywords:
Small intestinal organoids;
Pathology;
Immunohistochemistry;
Immunofluorescence;
Mouse
- From:
Acta Laboratorium Animalis Scientia Sinica
2017;25(5):513-518
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a small intestinal organoid culture system as an in vitro study model of intesti-nal epithelial cells, and to explore the relevant pathological detection techniques and provide a convenient platform for in vitro study of various intestinal diseases. Methods The mouse intestinal epithelium was isolated and cultured into or-ganoids to simulate the growth and development of intestinal epithelium in vitro. The proliferation and differentiation signals were detected by immunohistochemistry and three-dimensional immunofluorescence technique. Results The culture system of the mouse small intestine epithelium was established. Immunohistochemical staining and three-dimensional immunofluo-rescence technique were successfully used to detect the growth and development of small intestinal organoids. Conclusions The successfully established mouse small intestinal organoid culture system and application of immunoassay technology will gradually become a most favorable technical means for studies of various intestinal diseases.
