Establishment of an anxiety-like gastric hypersensitivity rat model of functional dyspepsia induced by a new method of sequential stress
10.3969/j.issn.1005-4847.2017.05.015
- VernacularTitle:新型序贯应激法致焦虑样胃高敏感性大鼠功能性消化不良模型的建立
- Author:
chun Fu JING
1
;
Jun ZHANG
;
Cheng FENG
;
yuan Yuan NIAN
;
de Bao YANG
;
Hao HU
;
ming Xiao SUN
;
yun Jian ZHENG
Author Information
1. 西安交通大学第二附属医院消化内科
- Keywords:
Sequential stress;
Anxiety;
Gastric hypersensitivity;
Functional dyspepsia;
Rat model
- From:
Acta Laboratorium Animalis Scientia Sinica
2017;25(5):551-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an anxiety-like gastric hypersensitivity ( GHS) rat model of functional dyspep-sia induced by new sequential stress. Methods Twenty-six male 1-day-old Sprague-Dawley rat pups were randomly di-vided into control and model groups ( n=13 in each group) . The model rats received sequential stress from postnatal day 2:neonatal maternal separation (NMS), acute gastric irritation (AGI) and restraint stress (RS). The control rats were reared freely with their mothers without undergoing the sequential stress. From postnatal 8th week all rats started to receive elevated plus maze ( EPM ) , open field test ( OF ) , abdominal withdrawal reflex ( AWR ) and electromyographic test ( EMG) . Results EPM and OF experiments depicted that the model rats showed obvious anxiety-like behavior ( P<0. 05 or P<0. 01). AWR and EMG tests exhibited that the model rats had elevated gastric hypersensitivity to gastric distention (P<0. 05 or P<0. 01). Conclusions An anxiety-like GHS rat model of functional dyspepsia can be successfully estab-lished with our new method of sequential stress.
