PM2.5 induces oxidative stress and histopathological changes in the uterus of rats
10.3969/j.issn.1005-4847.2017.05.010
- VernacularTitle:PM2.5对大鼠子宫组织形态及氧化应激效应的影响
- Author:
quan Feng ZHANG
1
;
heng En DONG
;
qiang Jian WANG
;
cheng Jin CAO
;
dong Xu GUO
;
dong Wei WU
Author Information
1. 新乡医学院公共卫生学院新乡市大气污染健康效应与干预重点实验室
- Keywords:
PM2. 5;
Rat;
Uterus;
Oxidative stress;
Histopathology
- From:
Acta Laboratorium Animalis Scientia Sinica
2017;25(5):524-527
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this work was to study whether PM2. 5 induces oxidative stress and histopatholog-ical changes in uterine tissue of rats. Methods Thirty 4-week-old female Sprague-Dawley rats were randomly divided into the control group (normal saline), the low dose of PM2.5 group (1. 5 mg/kg) and the high dose of PM2.5 group (37. 5 mg/kg) . After exposed to PM2. 5 for 10 days, the rats were sacrificed to examine the histopathological changes in uterine tissues using H&E staining. The contents of SOD, GSH, MDA and LDH were also determined in the uterine tissues. Results Compared with the control group, PM2. 5 caused changes in the uterine structure, showing a thinner endometrial epithelium and reduction of stromal cells and blood vessels. The assessment of oxidative stress parameters showed that the levels of MDA and LDH in the high dose group were (6. 53 ± 1. 24) nmol/mg prot and (265. 62 ± 24. 65) U/g prot, significantly higher than those in the control group ( P<0. 05 ) . Compared with the control group, the levels of SOD and GSH in the high dose and low dose groups were significantly decreased (P<0. 05). Conclusions PM2.5 exposure can cause damages in the rat uterus by inducing oxidative stress.
