Establishment and evaluation of a guinea pig model of itching
10.3969/j.issn.1005-4847.2017.05.011
- VernacularTitle:豚鼠皮肤瘙痒动物模型的建立及评价
- Author:
zhong Ben MI
1
;
bin Wu YANG
;
ling Xiao LUO
;
Yi WANG
;
Juan HU
;
hong Jia XU
;
fang Xing QIAO
Author Information
1. 重庆市中药研究院实验动物研究所
- Keywords:
Histamine;
4-aminopyridine;
Guinea pig;
Itching model
- From:
Acta Laboratorium Animalis Scientia Sinica
2017;25(5):528-533
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the optimal doses of histamine and 4-aminopyridine (4-AP) in the establish-ment of guinea pigs models of itching, and to establish a new guinea pig model of itching. Methods The central composite design-response surface method was used to arrange the experiment. In the experiment different pruritus agents were hypo-dermically injection of 0. 5 mL in the depilated area, and the scratching incubation period and scratching number in 30 mi-nutes were counted after the injection. The guinea pig itching model was evaluated by observing the behavioral changes of guinea pigs and measuring the levels of histamine and interleukin-6 in the blood. Results The behavioral experiments found that the scratching frequency in the the combination group was significantly higher than the histamine group and 4-AP group (P<0. 01). The itching latency of the combination group was significantly shorter than that of the histamine group and 4-AP group (P<0. 01). Compared with the control group, the histamine concentrations of the combination group and histamine group were significantly increased ( P<0. 05 or P<0. 01 ) , and the level of the combination group was lower than that of the histamine group (P<0. 05). Compared with the control group, the serum IL-6 concentrations of histamine group, 4-AP group and combination group were significantly higher (P<0. 01 or P<0. 05), and those in the combination group were significantly higher than the histamine and 4-AP groups. Compared with the control group, pathologic examina-tion showed proliferation of inflammatory cells in all model groups, and the reaction of the combination group was more ob-vious. Conclusions The optimal conditions used in this experiment are easy to achieve and have good reproducibility in the establishment of a guinea pig model of itching.
