Effect of mifepristone on ciliary beat frequency and estrogen and progesterone receptor expression levels in human fallopian tubes
10.3969/j.issn.1674-8115.2017.10.019
- VernacularTitle:米非司酮对人输卵管上皮纤毛摆动频率及雌、孕激素受体的影响
- Author:
jing Jiang YUAN
1
;
hong Wei ZHAO
;
Duo ZHANG
;
ying Xiao HE
;
Jian ZHANG
Author Information
1. 上海交通大学医学院附属国际和平妇幼保健院妇科
- Keywords:
mifepristone;
ciliary beat frequency;
human fallopian tube;
estrogen receptor;
progesterone receptor
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2017;37(10):1402-1406
- CountryChina
- Language:Chinese
-
Abstract:
Objective · To investigate ciliary beat frequency (CBF), and estrogen and progesterone receptor expression levels of human fallopian tubes after mifepristone treating in vitro. Methods · Human fallopian tube mucosa explants (n=25) were treated with different concentrations of mifepristone (0.1,1 and 10 μmol/L) or progesterone (10 μmol/L) separately, or both mifepristone and progesterone. After 24 h of treatment, CBF was measured. Quantitative real-time PCR and immunohistochemistry were used to research the expression of estrogen receptor-α (ERα) and progesterone receptor (PR) of human fallopian tubes after mifepristone treating. The ultrastructure of epithelial cells of fallopian tube after mifepristone (10 μmol/L) treating were observed with transmission electron microscopy. Results · The CBF at the concentrations of 0.1, 1 and 10 μmol/L was not affected by mifepristone (P=0.728,P=0.405 and P=0.941). The CBF decreased markedly in the group of 10 μmol/L progesterone compared to control group (P=0.000). Mifepristone (0.1,1 and 10 μmol/L) dose dependently antagonized the progesterone-induced CBF decrease (P=0.484, P=0.000 and P=0.000). Mifepristone upregulated the expression levels of ERα and PR in the fallopian tubes, but the ultrastructure of the cilia had no significant change. Conclusion · Mifepristone acts as progesterone antagonist in the human fallopian tube, which may explain the tubal contraceptive mechanism when used as an emergency contraceptive.
