Optimization of aqueous extraction for polysaccharides from Astragali Radix and the in vitro antitumor activity
10.3969/j.issn.1001-1528.2017.10.011
- VernacularTitle:黄芪多糖水提工艺的优化及其体外抗肿瘤活性
- Author:
jun Li YAN
1
;
Tao HONG
;
ling Fu WANG
;
Jian LI
;
Shuo TIAN
;
Duo ZHANG
;
Yuan GAO
;
han Jiang LUO
Author Information
1. 哈尔滨商业大学药学院
- Keywords:
polysaccharides from Astragali Radix;
aqueous extraction;
in vitro antitumor activity;
NCI-H460 cells
- From:
Chinese Traditional Patent Medicine
2017;39(10):2045-2049
- CountryChina
- Language:Chinese
-
Abstract:
AIM To optimize the aqueous extraction for polysaccharides from Astragali Radix and to evaluate the in vitro antitumor activity.METHODS With extraction temperature,extraction time and solid-liquid ratio as influencing factors,extraction rate of polysaccharides as an evaluation index,the extraction was optimized by uniform design.The effect of polysaccharides on the proliferation of non-small cell lung cancer NCI-H460 cells,the apoptosis rate and cell cycle of NCI-H460 cells,and the expressions of Caspase-3,Bax and Bcl-2 were detected by MTT assay,flow cytometry and Western blot,respectively.RESULTS The optimal conditions were determined to be 100 ℃ for extraction temperature,1 h for extraction time,and 1 ∶ 35 for solid-liquid ratio,the extraction rate of polysaccharides was 3.62％.Compared with the control group,the proliferation of NCI-H460 cells was significandy inhibited in a dose-dependent manner (P ＜ 0.01),the S phase ratio,early apoptosis rate,late apoptosis rate and total apoptosis rate were markedly increased (P ＜ 0.01),and the Caspase-3 expression and Bax/Bcl-2 ratio were also obviously increased (P ＜ 0.01) in the polysaccharides group.CONCLUSION This fast,stable and reliable method can be used for the aqueous extraction for polysaccharides from Astragali Radix,which can significantly inhibit the proliferation of NCI-H460 cells and induce apoptosis of NCI-H460 cells.
