Simultaneous determination of four flavonoids in Rubus chingii by MIPs-HPLC
10.3969/j.issn.1001-1528.2017.10.023
- VernacularTitle:MIPs-HPLC法同时测定覆盆子中4种黄酮
- Author:
juan Ya MA
1
;
ting Wen BAI
;
fang Xiao ZHU
;
ping Li HUANG
;
hui Yi XIE
Author Information
1. 江西中医药大学
- Keywords:
Rubus chingii Hu;
tannins;
lilsonide;
quercetin;
kaempferol;
molecular imprinted polymers (MIPs);
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(10):2097-2101
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a molecular imprinted polymers (MIPs)-HPLC method for the simultaneous content dertermination of four flavonoids in Rubus chingii Hu.METHODS Fe3O4 magnetic MIPs was added into ethyl acetate fraction solution of R.chingii to prepare mixed MIPs.The analysis of mixed MIPs methanol eluent was performed on a 30 ℃ thermostatic Diamonsil C18 column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 266 nm.RESULTS Tannins,lilsonide,quercetin and kaempferol showed good linear relationships within the ranges of 2.4-1 232.0 μg (R2 =1)、7.1-3 648.0 μg (R2 =0.999 9)、4.7-4 840.0 μg (R2 =0.999 9)、4.8-2 440.0 μg (R2 =0.999 9),whose average recoveries were 98.94％,99.33％,99.26％ and 98.67％ with the RSDs of 2.04％,1.40％,1.76％ and 1.75％,respectively.CONCLUSION This accurate and reliable method eliminates impurity interference,which can be used for the content determination of flavonoids in R.chingii.
