Differential expression of estrogen receptor GPR30 in main supporting ligaments of patients with pelvic organ prolapsed
10.3969/j.issn.1674-8115.2017.10.013
- VernacularTitle:盆底器官脱垂患者主要支持韧带中雌激素受体GPR30表达的研究
- Author:
Le KANG
1
;
Li CHEN
;
xia Cai LI
;
Xia CHEN
;
ping Zhong CHENG
Author Information
1. 同济大学附属杨浦医院妇产科
- Keywords:
pelvic organ prolapse;
estrogen receptor;
GPR30;
collagen;
elastin
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2017;37(10):1376-1379
- CountryChina
- Language:Chinese
-
Abstract:
Objective · To investigate the expression levels of estrogen receptor GPR30, elastin, collagen Ⅰ (Col Ⅰ ), and Col Ⅲ in the uterus round ligament and the cardial ligament in patients with pelvic organ prolapse (POP) and main components in extracellular matrix (ECM). Methods · 22 patients with POP (the POP group) and 10 patients with cervical intraepithelial neoplasia (CIN) Ⅲ (the control group) were enrolled. Hysterectomy specimens were collected and expressions of GPR30, elastin, Col Ⅰ and Col Ⅲ in the uterine round ligament and the cardial ligament tissues were detected with Western blotting and immunohistochemistry. Results · Western blotting and immunohistochemistry results showed that the expressions of GPR30, elastin, Col Ⅰ and Col Ⅲ in the uterine round ligament and the cardial ligament tissues were significantly lower in the POP group than in the control group. The differences were statistically significant (P<0.05). Conclusion · The occurrence of POP may be associated with estrogen receptor deficiency and decreased contents of collagen and elastin in relevant tissues. Whether GPR30 is involved in the synthesis, secretion and degradation of ECM major components in pelvic support tissues needs further investigation.
