The differential expressions and targeted regulation of miRNAs in the prefrontal cortex of rats with alcohol dependence
10.3760/cma.j.issn.1674-6554.2017.09.003
- VernacularTitle:酒精依赖大鼠前额叶皮质miRNAs差异表达及靶向调控分析
- Author:
Feng XIN
1
;
Lijuan YAN
;
Liping LIU
;
Xinxin ZHOU
;
Jian HU
Author Information
1. 哈尔滨医科大学附属第一医院精神科
- Keywords:
Alcohol dependence;
Prefrontal cortex;
MicroRNAs;
Target genes
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2017;26(9):782-787
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between alcohol dependence and miRNAs expression,and the possible mechanism of miRNAs in the process of alcohol dependence,Methods Alcohol dependence model rats were established by intragastric administration.The successful model rats of ethanol dependent were randomly divided into continuous alcohol group(n=3) and withdrawal group(n=3).The expression of miRNAs in the the prefrontal cortex of rats were detected using gene chip in rats of continuous alcohol group,withdrawal group and normal control group.The target genes for miRNAs with differentially expressed were analyzed by bioinformatics methods.Results The results showed that the expression of miR-532-3 p,miR-20b-3p,miR-411-3p,miR-16-3p,miR-299a-3p was up-regulated,and the expression miR-218b,miR-182,miR-122-5p,miR-1912-3p,miR-551 b-3p was down-regulated in the continous drinking group compared with those in the control group.Compared with control group,miR-292-5p up-regulated expression and miR-96-5p,miR-182,miR-144-5p reduced expression in alcohol group.Compared with alcohol withdrawal group,miR-1298,miR-292-5p,miR-122-5p,miR-3065-5p,miR-55 1b-3p up-regulated expression and miR-382-3p,miR-551b-5p reduced expression in continued drinking group.These differentially expressed of miRNAs may targeted to NEGR1,CDC42,Bcl2,CACNA1C,BDNF,ALDH and IGF-1 genes.Those played a role in the pathogenesis of alcohol dependence.Conclusion MiRNAs may regulate the expression of target genes through interaction and participate in the pathogenesis of alcohol dependence.
