Effect of Shenqi Fuzheng injection on invasion of MHCC97L cells based on RNA interference technology
10.3969/j.issn.1009-99905.2017.07.001
- VernacularTitle:RNA干扰技术探讨参芪扶正注射液对MHCC97L细胞侵袭力的影响
- Author:
heng Xiao CHEN
1
;
Bei HE
;
long Hui LI
;
Rui HU
;
Lu LI
;
Xin WANG
;
shun Tang WANG
;
qing Nai LI
;
guo Zhi DING
Author Information
1. 北京中医药大学东直门医院外一科 北京 100700
- Keywords:
Liver tumor;
Shenqi Fuzheng injection;
VEGF;
Invasiveness
- From:
Chinese Journal of Current Advances in General Surgery
2017;20(7):505-510
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of Shenqi Fuzheng injection on the expression of VEGF gene in human hepatocellular carcinoma cell line MHCC97L,and to elucidate the effect of Shenqi Fuzheng injection on tumor invasiveness,and to validate it through siRNA technology.Methods:After 24 hours of addition of Shenqi Fuzheng Injection (MHC) in the culture system of MHCC97L,Real-time PCR and Western-blot were used to detect the expression of MMP-9 and MMP-9 in the control group (Shenqi Fuzheng),negative control group (NaCI) And the expression of VEGF and RNA in the blank control group,and to determine the effect of Shenqi Fuzheng injection on the expression of VEGF and RNA.The effect of Shenqi Fuzheng Injection on the invasiveness of MHCC97L cells was detected by Transwell assay.The lentiviral vectors of MHCC97L cells were transfected into 293T cells and transfected into MHCC97L cells.The transfected cells were transfected into 293T cells by Lipofectamine 2000,and transfected into MHCC97L cells.The Effect of Shenqi Fuzheng Injection on the Invasion of VEGFi Tumor by Transwell.Results:Shenqi Fuzheng injection could significantly decrease the expression of VEGF mRNA and protein in normal MHCC97L cells (P＜0.05),and decrease the invasiveness of tumor cells (P＜0.05).VEGF RNA interference was successful.After intervention,Shenqi Fuzheng injection could not decrease the invasiveness of MHCC97L tumor (P＞0.05).Conclusion:Shenqi Fuzheng injection can reduce the invasion of MHCC97L cells by inhibiting the expression of VEGF gene.
