Effects of SRSF9/SRp30c on proliferation and migration abilities of glio-ma cells by regulating GRβ
10.3969/j.issn.1000-4718.2017.10.015
- VernacularTitle:SRSF9/SRp30c通过调控GRβ对胶质瘤细胞增殖和迁移的影响
- Author:
Yan LIU
1
;
mo A SHAO
;
Ying YIN
;
Zheng LI
;
Rui ZHANG
;
Da ZONG
;
Jian ZOU
;
ling Ya HU
Author Information
1. 无锡卫生高等职业技术学校
- Keywords:
Glioma;
Glucocorticoid receptor β;
Serine-arginine-rich splicing factor 9/serine-arginine-rich protein 30c;
Cell proliferation;
Cell migration
- From:
Chinese Journal of Pathophysiology
2017;33(10):1825-1830
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the expression of serine-arginine-rich splicing factor 9/serine-arginine-rich protein 30c (SRSF9/SRp30c) and glucocorticoid receptor β(GRβ) in the glioma cells and the relationship of them. METHODS:Small interfering RNA ( siRNA) was used to knock down the expression of SRSF9 in the U87 cells.Short hairpin RNA ( shRNA) derived from lentivirus was used to establish U 87 stable knockdown cell line .Fluorescence micros-copy was used to observe and detect transfection efficiency .The expression of Grβand SRSF9/SRp30c at mRNA and pro-tein levels was determined by RT-qPCR and Western blot .The cell viability , colony formation ability and migration ability were measured by CCK-8 assay, colony formation assay and wound healing experiment .RESULTS:The mRNA and pro-tein levels of SRSF9/SRp30c and Grβin the U87 cells were both down-regulated after knockdown of SRSF9 (P<0.05). Fluorescence microscopic observation showed that a stable cell line was constructed successfully , and the transfection effi-ciency exceeded 80%.After knockdown of SRSF9 expression in the U87 cells, the cell viability and colony formation abili-ty were reduced (P<0.05).The migration ability was weakened significantly after SRSF9 was knocked down (P<0.05). CONCLUSION:SRSF9/SRp30c may promote the proliferation and migration of the glioma cells by regulating GRβ.
