Effect of exogenous hydrogen sulfide on lipophagy in mouse primary hep-atocytes
10.3969/j.issn.1000-4718.2017.10.028
- VernacularTitle:外源性硫化氢对小鼠原代肝细胞内脂质自噬分解的影响
- Author:
gang Hong WANG
1
;
jing You ZHANG
;
shen Chao HUANGFU
;
liang Ming CHEN
;
Jun WANG
;
dong Dong WU
;
xing Zhen XIE
;
zhang Yan LI
;
ling Ai JI
Author Information
1. 河南大学基础医学院
- Keywords:
Hydrogen sulfide;
Lipolysis;
Hepatocytes;
Autophagy
- From:
Chinese Journal of Pathophysiology
2017;33(10):1901-1905
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To evaluate the effect of exogenous hydrogen sulfide ( H2 S) from GYY4137 on lipophagy in mouse primary hepatocytes .METHODS:The C57BL/6 mouse primary hepatocytes isolated and cultured by 2-step in situ perfusion method were divided into 4 groups:the cells in control group were incubated with normal medium; the cells in model group were incubated with 1.2 mmol/L oleic acid (OA) for 48 h;the cells in H2S group or propargylglycine (PAG) group were incubated with 1.2 mmol/L OA for 48 h followed by serum-free phenol red-free RPMI-1640 medium which con-tained 1 mmol/L GYY4137 or 200 μmol/L PAG for 6 h.The cells were collected to conduct immunofluorescence staining of LC3 and photography under fluorescence microscope , phase-contrast microscope or transmission electron microscope . The protein expression of LC 3-Ⅰ/Ⅱin the hepatocytes was determined by Western blot .RESULTS:In contrast with the model group, the fluorescent particles of LC3, the protein expression of LC3, the number of autophagic lysosome and vacuoles in hepatocytes in H 2 S group increased .CONCLUSION: In steatosis hepatocytes , exogenous H 2 S promotes the lipophagy .
