Effect of metabolites of Clostridium butyricum, butyric acid and hydro-gen, on acute gastric mucosal lesion
10.3969/j.issn.1000-4718.2017.10.029
- VernacularTitle:酪酸梭菌代谢产物丁酸和氢气对急性胃黏膜损伤的作用
- Author:
dan Jin WANG
1
;
ning Wan QIAO
;
Jing ZHU
;
yang Yao FU
;
li Wan HU
;
jie Ren DONG
;
rui Kai HONG
;
li Jia ZHANG
;
yan Fang WANG
Author Information
1. 温州医科大学检验医学院、生命科学学院
- Keywords:
Butyric acid;
Hydrogen;
Acute gastric mucosal lesion;
Inflammation;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2017;33(10):1906-1911
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the antiulcer effect of butyric acid and hydrogen , the main metabolites of Clos-tridium butyricum (C.butyricum), and to explore the underlying mechanism .METHODS: The mouse model of acute gastric mucosal lesion was prepared by gavage with ethanol .The mice were randomly divided into 4 groups:normal group , model group , butyric acid group and hydrogen group .The mice in butyric acid group and hydrogen group were given buty-rate and hydrogen prior to model establishment , respectively .Macroscopic observation of the pathological changes in gastric tissues was performed to evaluate the effect of the 2 metabolites of C.butyricum.Meanwhile, the mRNA expression levels of inflammatory factors, such as IL-12, RAN1 and MCP-1, were determined by RT-qPCR.The expression levels of apopto-sis-related proteins Bcl-2 and Bax were detected by immunohistochemical staining .RESULTS:The macroscopic observa-tion found that butyrate , not hydrogen , protected gastric mucosa .HE staining also showed that butyrate significantly attenu-ated the pathological damage of the gastric mucosa induced by ethanol .Compared with model group , the mRNA levels of inflammatory factors IL-12, RAN1 and MCP-1 in butyrate group significantly decreased (P<0.01).In butyrate group, the protein level of Bax was obviously decreased compared with model group (P<0.01), while the protein level of Bcl-2 was significantly increased ( P<0.01 ) .CONCLUSION: The gastric mucosa protective metabolite of C.butyricum may be butyric acid , not hydrogen .Butyric acid protects the gastric mucosa against ethanol-induced lesion by inhibiting the inflam- mation and reducing the expression ratio of Bax/Bcl-2.
