Experimental study on immatural dendritic cells infected by HIV-1 pseudovirus
10.3969/j.issn.1000-484X.2017.10.001
- VernacularTitle:HIV-1假病毒感染未成熟树突状细胞实验研究
- Author:
Na ZHU
1
;
Li Xue LIU
;
Ni QIU
;
Ting Jie ZHANG
;
Pin Shi WU
;
Yuan Ti LI
;
Yan GUO
;
Chang LI
;
Yi Ning JIN
Author Information
1. 青岛市中心医院药学部
- Keywords:
HIV-1 pseudovirus;
Immatural dendritic cell;
Infection experiment
- From:
Chinese Journal of Immunology
2017;33(10):1441-1446
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct HIV-1 pseudovirus containing enhanced green fluorescent protein ( EGFP ) gene. To understand the interaction between the virus and the cells. Methods: HIV-1 pseudovirus containing EGFP gene was constructed by lentiviral packaging systems, and its EGFP gene was amplified using RT-PCR. The level of genomic integration and transcription of HIV-1 pseudovirus containing EGFP gene were detected on iDCs infected with HIV-1 pseudovirus. At the same time, research on expression of the EGFP gene in iDCs infected with HIV-1 pseudovirus was performed. Results:The EGFP gene of HIV-1 pseudovirus was detected through RT-PCR. The EGFP gene was identified in iDCs infected with HIV-1 pseudovirus through PCR and RT-PCR. The EGFP was observed in iDCs infected with HIV-1 pseudovirus under fluorescence microscopy. Conclusion: HIV-1 pseudovirus containing EGFP gene has been successfully produced. The HIV-1 pseudovirus that we constructed can infect iDCs,then its RNA can integrate into the genome of iDCs in the way of reverse transcription,and the EGFP gene could express in the iDCs after infected with HIV-1 pseudovirus.
