microRNA-155 inhibits immuno-inflammatory reaction of foam cells by targeting MyD88
10.11958/20170719
- VernacularTitle:microRNA-155通过MyD88抑制泡沫细胞免疫炎症反应
- Author:
yang Xiang JING
1
;
ling Yun LIU
;
ping Li LU
;
Xue LIANG
;
zhao En LIU
Author Information
1. 天津医科大学第二医院心脏科
- Keywords:
microRNAs;
atherosclerosis;
myeloid differentiation factor 88;
foam cells;
miR- 155;
ox- LDL;
inflammatory factor
- From:
Tianjin Medical Journal
2017;45(10):1013-1016
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of microRNA-155 (miR-155) on immuno-inflammatory reaction of foam cells by targeting MyD88 and the possible mechanism. Methods RAW264.7 macrophages were cultured in vitro and transfected with miR-155 mimics, miR-155 inhibitor, MyD88 siRNA and their negative control respectively, then ox-LDL stimulation was given to build foam cell model. The expression of MyD88 in foam cells was detected by RT-qPCR and Western blot assay. Moreover, the expression levels of interleukin (IL)-10, TGF-β1 and MCP-1 in supernatant were determined by ELISA. Results After being transfected with miR-155 mimics, the mRNA level of MyD88 remained unchanged compared with that of control group (P>0.05). The protein level of MyD88 decreased significantly (P<0.05), and the expression levels of IL-10, TGF-β1 and MCP-1 in supernatant also decreased significantly (P<0.05). After being transfected with miR-155 inhibitor, the mRNA level of MyD88 remained unchanged compared with that of control group (P>0.05). The expression levels of MyD88 protein and inflammatory cytokines increased significantly (P<0.05). After being transfected with MyD88 siRNA, the expression levels of MyD88 mRNA and protein decreased significantly, and the expression levels of inflammatory cytokines also decreased significantly (P<0.05). Conclusion miR-155 can negatively regulate inflammation by targeting MyD88 through the inhibition of translation.
