Production and application of monoclonal antibody against major histocompati-bility complex class Ⅰrelated chain A and B
10.3969/j.issn.1000-484X.2017.10.015
- VernacularTitle:抗主要组织相容性相关蛋白MICA/B的单克隆抗体制备及应用
- Author:
Fang TIAN
1
;
Guang Wei LUO
;
Li Xu GUO
;
Jing GUO
;
Zhi Qi LUO
;
Peng Hui WANG
;
Zhou Yi ZOU
Author Information
1. 中南大学基础医学院医学免疫学系
- Keywords:
MICA;
MICB;
Monoclonal antibody;
Hybridoma cells
- From:
Chinese Journal of Immunology
2017;33(10):1514-1519
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Generation and identification of hybridoma to produce monoclonal antibody against MICA/B. Methods:SP2/0 cells were fused with murine splenocyte immunized with recombinant protein rMICA?012 to get hybridoma cell lines. The titer of the monoclonal antibody produced by 9B10 cell line was determined by ELISA and its specificity was tested by Western blot,Luminex mutiplex microsphere-based immunoassay and immunofluorescence assay. Results:Six hybridoma cell lines were selected by ELISA screening test. The minimum reaction concentration of mAb 9B10 was 0. 02 ng/μl,and the specificity of mAb 9B10 was determinated by Western blot,Luminex mutiplex microsphere-based immunoassay and immunofluorescence. Conclusion:The monoclonal antibody 9B10 was available to apply for the detection of MICA and MICB expression.
