Genotyping of hepatitis C virus by PCR-fluorescent probe in Qingyang area
10.3969/j.issn.1000-484X.2017.10.017
- VernacularTitle:PCR-荧光探针法检测庆阳地区丙型肝炎病毒基因型
- Author:
Juan Juan HOU
1
;
Feng Zhi ZHANG
;
Ping Ji ZHANG
;
Yue Wei XI
;
Jun Hong ZHENG
;
Yan Hai ZHAO
;
Juan LI
Author Information
1. 庆阳市人民医院检验科
- Keywords:
PCR-fluorescent probe method;
Qingyang;
Hepatitis C virus;
Genotype
- From:
Chinese Journal of Immunology
2017;33(10):1526-1529
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the genotyping of hepatitis C virus by PCR-fluorescent probe in Qingyang area,and to evaluate the performance of PCR-fluorescent probe. Methods:The clinical data and peripheral venous blood of patients with HCV were collected (n=289). PCR-fluorescent probe was used to detect the genotype and HCV RNA of hepatitis C virus,and compare with PCR reverse dot blot,RT nested-PCR. Results:Among 289 samples detected by PCR-fluorescent probe,the rate of genotyping of hepatitis C virus was 99. 3%(287/289),and 139 for 1b(48. 1%),136 for 2a(47. 1%),7 for 3a(2. 4%),5 for 3b(1. 7%),2 for unknow(0. 7%). The specificity and efficiency was 100%,better repeatability,consistent with PCR reverse dot blot and RT nested-PCR(98. 2%,P>0. 05). The ALT,AST,PLT and HCVRNA(lg)for 1b patients was higher than 2a(P<0. 05). Conclusion:Multi-genotype distribution of HCV was revealed in the hepatitis C patients of Qingyang,1b and 2a were the main genotypes,and the ratio was equal,2a was increased,1b was declined. The sensibility and specificity was higher for PCR-fluorescent probe,and could be used in clinic.
