Inhibitory effect of curcumin on human breast cancer cell line SKBR3 and its mechanism
10.3969/j.issn.1005-1678.2017.10.006
- VernacularTitle:姜黄素对人乳腺癌SKBR3细胞株的抑制作用及机制研究
- Author:
ke Jia YING
1
;
Yu YE
;
long Di FANG
Author Information
1. 杭州市红十字会医院 普外科
- Keywords:
curcumin;
SKBR3 cells;
telomerase;
caspase-3;
apoptosis
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(10):15-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of curcumin on proliferation, apoptosis, Caspase-3 activity and telomerase activity of chronic granulocytic breast cancer SKBR3 cell line, and to investigate the mechanism of curcumin. Methods The cell growth curve was drawn by MTT;the apoptotic cells were detected by Annexin, V and PI double staining; the expression of Caspase-3 was detected by Westernblot; the activity of telomerase was detected by TRAP-PCR silver staining. Results Curcumin could significantly inhibit the proliferation of SKBR3 cells in a time and concentration dependent, has a certain effect on reducing the telomerase activity of SKBR3 cells can enhance the expression of Caspase-3 induced apoptosis. Conclusion Curcumin can effectively inhibit the proliferation of SKBR3 cells, increase the synthesis of Caspase-3 protein, inhibit the activity of telomerase and mediate the apoptosis of SKBR3 cells.
