Isobaric tag for relative and absolute quantitation-based proteomics analysis of synovial fluid from osteoarthritic knee
10.3760/cma.j.issn.1007-7480.2017.09.009
- VernacularTitle:应用同位素标记相对和绝对定量蛋白质组学方法筛选骨关节炎患者关节液差异蛋白
- Author:
Qiang JIAO
1
;
Lei WEI
;
Xiaochun WEI
;
Zhiqiang ZHANG
;
Kun YIN
;
Pengcui LI
;
Li GUO
;
Yanxia YAN
;
Jiangong LU
Author Information
1. 山西医科大学第二医院骨科 骨与软组织损伤修复实验室
- Keywords:
Osteoarthritis;
Isotope labeling;
Proteomics;
iTRAQ
- From:Chinese Journal of Rheumatology
2017;21(9):622-627
- CountryChina
- Language:Chinese
-
Abstract:
Objective We performed comprehensive proteomic analyses of synovial fluid by using the isobaric tags for relative and absolute quantitation (iTRAQ) method and LC-MS/MS, and searched for candidate biomarkers for osteoarthritis. Methods Synovial fluid was collected from patients with late-stage OA, earlystage OA and the control group. Molecular variations were detected by the iTRAQ method. T test was used for statistical analysis. Results Using the iTRAQ method, we identified 1 283 proteins from synovial fluid of patients with osteoarthritis, of which 268 proteins were not reported previously. There were 72 proteins upregulated and 249 proteins down-regulated between moderate OA group and controls. One hundred and twentyeight proteins were up-regulated and 141 proteins were down-regulated between severe OA group and controls.One hundred and ninety-two proteins were up-regulated and 76 proteins were down-regulated between severe OA group and moderate OA group. Eight proteins were found to be up-regulated in the three groups.Conclusion This is an in-depth analysis of the synovial fluid proteome from patients with osteoarthritis by iTRAQ method. The catalog of proteins generated in this study will further expand our knowledge regarding the pathophysiology of osteoarthritis and help us in identifying good biomarkers for early diagnosis.
