Inhibitory effect of Gallnut on Candida albicans biofilm
10.3969/j.issn.1005-1678.2017.10.003
- VernacularTitle:五倍子对白色念珠菌生物被膜的抑制作用
- Author:
Jia Ming ZHANG
1
;
Lu Lu JIANG
;
Jie Ming XIE
Author Information
1. 辽宁师范大学生命科学学院 辽宁省生物技术与分子药物研发重点实验室
- Keywords:
Gallnut;
Candida albicans;
biofilm
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(10):6-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effect of Gallnut on the biofilm of Candida albicans. Methods MTT assay was used to determine the inhibitory effect of Gallnut on biofilm formation and mature biofilm. RT-PCR was used to determine the effect of Gallnut on the expressions of ALS1 and CPH1 genes. Results Gallnut has a stronger inhibitory effect on Candida albicans biofilm formation, the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) for inhibition of biofilm formation both are 128mg/mL. Gallnut also has a strong inhibitory effect on the mature biofilm of Candida albicans, the minimum inhibitory concentration (MIC) is 256mg/mL. Gallnut could significantly reduce the gene expressions of ALS1 and CPH1. Compared with the control group, 64 mg/mL Gallnut act on Candida albicans after 16h, the gene expression of ALS1 and CPH1 decreased by 79.2% and 82.0% respectively. Conclusion Gallnut has a significant inhibitory effect on the formation and maturation of Candida albicans biofilm. The inhibitory effect of Gallnut on the adherence and invasion of Candida albicans is mainly through inhibiting the expressions of ALS1 and CPH1 genes.
