SFRP5 gene silencing promotes proliferation and migration of human pancreatic cancer cell line PANC-1
- VernacularTitle:SFRP5基因沉默促进人类胰腺癌细胞系PANC-1增殖与迁移
- Author:
rui Bao LIU
1
;
na Yong WU
;
ping Hai WANG
;
Hui ZHANG
;
guo Jian PAN
;
ce Wen ZHOU
Author Information
1. 兰州大学第一临床医学院
- Keywords:
lentiviral;
pancreatic neoplasms;
cell proliferation;
migration
- From:
Basic & Clinical Medicine
2017;37(10):1434-1439
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of lentivirus-mediated shRNA silencing SFRP5 on proliferation,in-vasion and migration of pancreatic cancer cell line PANC-1. Methods A SFRP5-knockdown recombinant plasmid was constructed and successfully transfected it into pancreatic cancer cell line PANC-1,blank plasmid transfection was treated as negative control and untreated cells as blank control group. The expression of SFRP5 at RNA and protein level in cell were detected by real-time PCR and Western blot, CCK-8 assay was applied to examine the effect of SFRP5 silencing on the proliferation, the cell migration of pancreatic cancer cell line PANC-1 was ana-lyzed by Transwell migration assay and cell scratch test was used to examine the cell invasion in PANC-1 cell. Results Stable transfected shRNA-SFRP5 cell of pancreatic cancer line was established successfully.The prolifera-tion capacity of SFRP5 group was significantly higher as compared to the negative control and blank control group by CCK8 assay(P<0.01).Similarly, cell invasion and migration of SFRP5 group were significantly higher compared to the negative control and blank control group(P<0.01). Conclusions SFRP5 lentiviral interference vectors can effectively decrease SFRP5 gene expression in PANC-1 cell of pancreatic cancer, thereby promoting cell proliferation,invasion and migration.
