5-Fluorouracil suppresses human hepatocellular carcinoma cell growth via promoting miR-22 expression
- VernacularTitle:5-氟尿嘧啶通过促进miR-22表达抑制肝癌细胞增殖
- Author:
qing Meng SUN
1
;
xing Yong DU
;
ze Zong LI
;
Lei YOU
;
Hong SHU
;
wen Zi LIU
Author Information
1. 中国医学科学院 北京协和医院 基本外科
- Keywords:
miR-22;
5-fluorouracil;
HCC;
chemotherapy
- From:
Basic & Clinical Medicine
2017;37(10):1368-1372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of 5-fluorouracil (5-FU) on miR-22 expression in human hepato-cellular carcinoma (HCC) cell lines and to elucidate the molecular mechanism of 5-fluorouracil for HCC chemo-therapy. Methods Real-time PCR analysis was conducted to determine the expression levels of miR-22 in HCC tissue specimens and HCC cell lines. The expression of miR-22 and pri-miR-22 (primary miR-22) was evaluated in HepG2 and Huh7 cells with 5-FU treatment by using real-time PCR and we also performed Western blot analysis to detect the protein level of HDAC4 in HCC cells with the same treatment. A rescue assay was employed by using 5-FU treatment in combination with miR-22 inhibitor(Anti-22) to further investigate the correlation among 5-FU, miR-22,and HCC cell growth. Results miR-22 expression depicted a significant downregulation in HCC tissues and cell lines (P<0.01). 5-FU treatment led to an augment of miR-22 expression in HepG2 and Huh7 cells(P<0.001) and resulted in a decrease of HDAC4 protein levels, which was verified as a direct target of miR-22 in HCC cells (P<0.01). Conclusions 5-FU has suppressive effect on HCC growth which could be potentially ex-plained by miR-22-mediated HDAC4 axis.
